神经生长因子滴眼液对兔眼LASIK术后角膜神经修复的影响
Effect of nerve growth factor eye drops on corneal nerve regeneration in rabbits after LASIK
目的 探讨神经生长因子(NGF)对兔眼准分子激光原位角膜磨镶术(LASIK)后角膜神经损伤再生修复的影响.方法 实验研究.将15只(30眼)薄瓣LASIK术后新西兰大白兔随机分为NGF组(NGF治疗)、阳性对照组(人工泪液玻璃酸钠滴眼液治疗)与阴性对照组(0.9%氯化钠溶液滴眼),每组各5只(10眼).利用共聚焦显微镜分别测量术前,术后1周、1个月、3个月中央角膜上皮下神经密度(SND)、上皮下神经数量及浅基质神经密度、浅基质神经数量,并进行比较.采用重复测量方差分析比较不同时间点及3组之间各神经参数的差异.结果 术前阴性对照组、阳性对照组、NGF治疗组SND分别为(10 801±3 331) μm/mm2、(11 619±3 932)μm/mm2、(12 299±2 622)μm/mm2,上皮下神经纤维数量分别为12.2±3.4、11.6±2.7、13.1±2.7,浅基质神经密度分别为(7258±1 242)μm/mm2、(8 148±2 462)μm/mm2、(8 984±1 526)μm/mm2,浅基质神经数量分别为8.5±1.4、8.9±2.6、10.1±2.1.与术前相比,3组所有的神经参数在术后1周均明显减少(P<0.01).与术后1周相比,NGF治疗组SND、浅基质神经密度在术后1个月均上升,而阴性对照组和阳性对照组的SND、浅基质神经密度在术后3个月才开始上升,差异有统计学意义(P<0.01);3组上皮下神经数量在术后3个月开始上升(P<0.05);NGF组和阳性对照组的浅基质神经数量在术后1个月上升(NGF组P<0.01,阳性对照组P<0.05),并且NGF组的浅基质神经数量在术后1个月恢复到术前水平.结论 NGF滴眼液对LASIK术后角膜神经的损伤再修复有明显促进作用.
更多Objective To investigate the effect of nerve growth factor (NGF) eye drops on central corneal nerve regeneration in rabbits after laser in situ keratomileusis (LASIK).Methods This was a experimental study.Fifteen New Zealand white rabbits were randomly assigned to one of three groups:NGF-treatment group,artificial tears hycosan-treatment group (positive control group),and normal saline-treatment group (negative control group) with five rabbits in each group,one drop every time and three times per day.The nerve density and the number of central corneal sub-basal and stromal cells were measured by confocal microscopy and were compared before surgery and at 1 week,1 month,and 3 months after surgery.Analysis of variance was used for comparing the differences at different time points and among the three groups.Results The respective preoperative sub-basal nerve densities (SNDs) in the negative control group,positive control group,and NGF treatment group were 10801±3331 μm/mm2,11 619±3 932 μm/mm2,and 12 299±2 622 μm/mm2.The number of corneal sub-basal nerve fibers were 12.2±3.4,11.6±2.7,and 13.1±9.7 in the respective groups.In the preoperative corneas,the comeal stromal nerve densities were 7 258±1 242 μm/mm2,8 148±2 462 μm/mm2,8 984±1 526 μm/mm2,and the number of corneal stromal nerve fibers were 8.5±1.4,8.9±2.6,10.1±2.1 in the respective groups.Compared with preoperative values,nerve parameters in all groups increased at 1 week postoperatively (P<0.01).Compared with 1 week postoperative,SNDs and corneal stromal nerve densities in the NGF group increased at 1 month,while the control groups increased at 3 months (P<0.01).The number of sub-basal corneal nerve fibers in all three groups increased at 3 months (P<0.05).The number of corneal stromal nerve fibers increased at 1 month compared with 1 week postoperative in the NGF group and the positive control group (P<0.05).However there were no significant changes in the number of corneal stromal nerve fibers between pre-and postoperative 1 and 3 months measurements.Conclusion NGF eye drops promote comeal nerve repair at different periods following LASIK in rabbit eyes.
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