广西巴马地区长寿家系口腔黏膜细胞端粒长度的研究
Investigation of telomere length in buccal cells in longevity families in Bama area of Guangxi
目的 探讨广西巴马地区壮族长寿家系口腔黏膜脱落上皮细胞端粒的长度及其潜在意义. 方法 应用实时荧光定量PCR方法测定巴马地区长寿家系(1250例)口腔黏膜脱落细胞的相对端粒长度,并与当地非长寿家系(556例)及外地区(广西平果)长寿家系(630例)比较. 结果 (1)在所有的研究人群(2436例)中,口腔黏膜脱落细胞端粒长度均与年龄呈负相关(R =-0.215,P=0.000),即年龄越大,端粒长度越短,而且这种变化不受性别的影响;(2)在巴马地区长寿家系中,各年龄段男性和女性的端粒长度均差异无统计学意义(均P>0.05),其他家系除个别年龄段外,也表现为类似的趋势;(3)巴马地区长寿家系65~年龄段(相当于长寿老人的第一胎子女)的端粒长度长于非长寿家系(1.969比1.622,P=0.004),同时,90~高龄段的端粒长度与非长寿家系65~年龄段的水平相当(1.662比1.622,P=0.955). 结论 巴马地区长寿家系口腔细胞的端粒长度随年龄的增大而缩短;端粒的长短有一定的遗传倾向,可能是该地区长寿的机制之一.
更多Objective To explore the telomere length (TL) in buccal cells and its possible implications for long-lived families of Zhuang nationality in Bama area of Guangxi.Methods Relative TL in buccal cells from Bama long-lived families (BLF,n=1250) was determined by real time PCR and compared between Bama non-long-lived families (BNLF,n=556) and Pingguo longlived families (PLF,n=630).All participants were Zhuang ethnic.Results The TL in buccal cells was negatively correlated with age (R=-0.215,P=0.000) and was independent of sex in all subjects (n=2436).There were no significant differences in TL between males and females in different of ages in BLF (all P>0.05).Similar tendency was observed in most but not all age groups in the two other families.The TL was significantly longer in BLF aged over 65 years (the first offspring of the longlived individual) than in BNLF aged over 65 years (1.969 vs.1.622,P=0.004) and the TL of BLF aged over 90 years was comparable to that of BNLF aged over 65 years (1.662 vs.1.622,P=0.955),which indicating that the offspring of long-lived individuals inherited longer TL from their parental generation.Conclusions Telomere length is shorten with aging in long-lived families in Bama area.The TL of BLF has a tendency of inheritance,which may be one of the mechanisms of longevity in Bama area.
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