胰岛素样因子3受体(LGR8)在生精细胞与精子顶体中表达
Insl3 receptor (LGR8)expressed on the acrosome of spermatocytes and sperm
使用免疫共沉淀及Western印迹,检测胰岛素样因子3(Insl3)受体(LGR8)在野生型(Wt)及Insl3基因敲除鼠(K0)睾丸表达情况.取胚胎18天(E18)和出生后(P)1、3、7、14、17、20、23、51和90天Wt及KO鼠睾丸及Wt小鼠精子,用免疫组化法检测LGR8表达.LGR8蛋白在所有年龄段小鼠睾丸间质细胞均为Wt着色强于KO鼠(均P<0.05).LGR8蛋白在小鼠睾丸的表达随年龄变化;除了在出生1天的Wt小鼠睾丸精细胞上强表达外,主要表达在青春期后、成熟的精细胞上及精子的顶体.
更多Immunoprecipitation and Western-blot were performed to detect the expression of LGR8 in the testes in wild type (Wt) and Insl3-knockout ( KO ) mice.Testis were harvested from WT and KO mice on embryonic day 18 (E18) and on postnatal(P) 1,3,7,14,17,20,23,51,and 90 days.LGR8 staining in Leydig cells was stronger in all Wt mice than that in KO mice( all P<0.05 ).LGR8 expression on spermatocytes varies with age.Strong stain on P1 Wt and postpubertal mice was concentrated in the developing and mature acrosome of spermatids
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