2型糖尿病肥胖患者SPARC表达差异性研究
Variations of SPARC in obese patients with type 2 diabetes mellitus
目的 观察正常人、单纯肥胖患者、单纯2型糖尿病患者、2型糖尿病伴肥胖患者血清中以及非糖尿病患者、2型糖尿病患者脂肪组织中富含半胱氨酸的酸性分泌蛋白(SPARC)表达水平差异情况.方法 ELISA法检测人血清中SPARC浓度,RT-PCR、Western印迹法、免疫荧光法检测人肠系膜脂肪组织中SPARC mRNA、蛋白的表达水平.结果 (1)单纯肥胖、单纯2型糖尿病及2型糖尿病伴肥胖患者与正常人比较,血清SPARC水平显著增加[(1 191.6±718.91、1 223.81±645.96、1 538.01±757.95对851.07±280.21)ng/L,P<0.05].(2)Pearson相关分析结果显示SPARC与稳态模型评估的胰岛素抵抗指数呈显著正相关(r=0.205,P<0.05).(3)2型糖尿病组脂肪组织中SPARC mRNA、蛋白表达水平高于对照组(P<0.05).结论 SPARC可能与肥胖、胰岛素抵抗及2型糖尿病的发生密切相关.
更多Objective To observe serum secreted protein acidic and rich in cysteine (SPARC) levels in normal subjects,obese subjects,patients with type 2 diabetes mellitus (T2DM),and obese patients with T2DM,as well as the difference of SPARC expression in mesenteric adipose tissue of subjects with and without T2DM.Methods Serum SPARC level was measured with the ELISA.RT-PCR,Western blot,and immunofluorescence were used to examine SPARC mRNA and protein expressions in mesenteric adipose tissue.Results (1) SPARC levels were higher in obesity,T2DM,and T2DM with obesity groups compared with normal group [(1 191.6 ± 718.91,1 223.81 ± 645.96,1 538.01 ± 757.95 vs 851.07 ± 280.21) ng/L,P<0.05].(2) Pearson correlation analysis showed that SPARC level was positively correlated with homeostasis model assessment for insulin resistance index (r =0.205,P< 0.05).(3) The expression levels of SPARC mRNA and protein in mesenteric adipose tissue of T2DM patients were higher than those of control subjects (P < 0.05).Conclusion SPARC is closely related to the development of obesity,insulin resistance,and type 2 diabetes mellitus.
More- 浏览:241
- 被引:5
- 下载:89
相似文献
- 中文期刊
- 外文期刊
- 学位论文
- 会议论文