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目的 观察长效胰升糖素样肽1(GLP-1)受体激动剂利拉鲁肽早期干预对高脂饮食诱导的追赶生长大鼠脂肪追赶生长的影响,并探讨其可能机制.方法 将64只SD大鼠分为4组:正常对照组、高脂饮食追赶生长组、追赶生长利拉鲁肽干预组和追赶生长利拉鲁肽及Exendin(9-39)共干预组.追赶生长各组大鼠先限食4周,再高脂饮食喂养4周,监测动物体重、能量摄入,分别在限食期末及追赶生长期末计算Lee指数,用双能X线吸收法检测大鼠身体组成,并处死部分动物取材,检测体脂分布及血脂水平.结果 在高脂追赶生长末期,利拉鲁肽干预大鼠体重、能量摄入、Lee指数、体脂含量,躯干脂肪比、内脏脂肪体脂比、甘油三酯、游离脂肪酸、低密度脂蛋白胆固醇均明显低于未干预的追赶生长大鼠(均P＜0.01),而利拉鲁肽及Exendin(9-39)共干预大鼠能量摄入、身体组成、体脂含量及血脂水平均与追赶生长大鼠无显著差异,但其体重略低于追赶生长大鼠且有统计学差异(P＜0.01).结论 利拉鲁肽早期干预能阻止高脂饮食诱导的追赶生长大鼠出现脂肪追赶生长现象,该作用主要通过GLP-1受体通路实现.

Objective To investigate whether liraglutide treatment can prevent catch-up fat in high-fat dietinduced catch-up growth rats,and to discuss the possible underlying mechanisms.Methods Sixty-four SpragueDawley rats were stratified into 4 groups:normal control group,catch-up growth (CUG) group,catch-up growth with liraglutide treatment (CUGL) group,and catch-up growth with liraglutide plus exendin (9-39) treatment (CUGLE) group.All the catch-up growth animals underwent a4 week high-fat diet re-feeding phase after a4 week food restriction phase.Body weight and food intake were measured every day.At the end of food restriction and re-feeding,body composition was measured via Dual energy X-ray absorptiometry.After the rats were sacrificed,body fat distribution and plasma lipid levels were determined.Results By the end of catch-up growth,body weight,energy intake,Lee' s index,fat body mass,visceral adipose tissue/body weight,subcutaneous adipose tissue/body weight,triglyceride,free fatty acid,low density lipoprotein-cholesterol levels were lower in CUGL rats compared with CUG rats (all P＜0.01).There were no significant differences in energy intake,body composition,fat body mass,and blood lipid levels between CUGLE rats and CUG rats,while the weight of CUGLE rats was significantly lower than CUG ones.Conclusions Liraglutide infusion protects rats with high-fat diet induced catch-up growth from catch-up fat mainly via a glucagon-like peptide-1 receptor dependent manner.