免疫相关性血细胞减少症患者骨髓CD4+T细胞中特异性转录因子Bcl-6、Foxp3和RORγt的表达
Changes of transcription factors Bcl-6, Foxp3 and RORγt in CD4+ cells in bone marrow of immuno-related hematocytopenia
目的 评价免疫相关性血细胞减少症(IRH)患者骨髓CD4+T细胞亚群中转录因子的异常.方法 收集2011年1月至2012年3月苏州大学附属第一医院血液科确诊的40例IRH患者的骨髓,采用流式细胞仪检测骨髓细胞的自身抗体.采用免疫磁珠分选IRH患者骨髓CD4+T细胞,采用实时荧光定量PCR检测骨髓细胞转录因子B细胞淋巴瘤6(Bcl-6)、叉状头/翅膀状螺旋转录因子3(Foxp3)和维甲酸依赖性孤儿受体(RORγt) mRNA表达水平,以同期25例缺铁性贫血患者作为正常对照组,以同期38例再生障碍性贫血(AA)患者作为病例对照组,进行比较.结果 IRH组在干/祖细胞(CD34+细胞)、粒系(CD15+细胞)、红系(GlyA+细胞)分别有67.5%(27/40)、65.0%(26/40)、75.0%(30/40)发现自身抗体,累及造血系统三系者为27.5% (11/40),两系者为52.5%(21/40),一系者为20.0% (8/40).IRH组Bcl-6、Foxp3的mRNA水平均高于正常对照组[2.243(0.854 ~4.544)比1.211(0.131 ~2.816),0.124(0.073 ~0.198)比0.071 (0.046 ~0.118),均P<0.05],Foxp3/Bcl-6比值与正常对照组比较差异无统计学意义(P>0.05).IRH组与AA组Foxp3mRNA表达量差异无统计学意义(P>0.05),但AA组RORγt mRNA表达水平显著高于IRH组[0.290(0.138~0.480)比0.133(0.068~0.189),P<0.01],IRH组Foxp3/RORγt比值高于AA组[0.975(0.483 ~1.416)比0.500 (0.240 ~0.795),P<0.01].结论 IRH是由抗造血细胞自身抗体所致造血功能衰竭症,可能与Bcl-6高表达有关,IRH免疫学发病机制与AA不同.
更多Objective To evaluate the possible mechanism of transcription factors B cell lymphoma 6 (Bcl-6),forkhead/winged helix transcription factor 3 (Foxp3) and retinoic acid related orphan receptor (RORγt) in CD4 + T cells for immuno-related hematocytopenia (IRH).Methods CD4 + T cells were harvested from 40 IRH patients,38 aplastic anemia subjects and 25 normal controls and separated by magnetic activated cell sorting (MACS).Then the expressions of transcription factors of Foxp3,RORγand Bcl-6 in CD4+ T cells were measured by real time fluorescent quantitative-polymerase chain reaction (QRT-PCR).Results Auto-antibody was detected on CD34 + cells (67.5% (27/40)),CD15 + cells (65.0% (26/40)),GlyA+ cells (75.0% (30/40)),auto-antibody involving three,two or one myeloid cell were detected in 27.5% (11/40),52.5% (21/40),20.0% (8/40) of IRH patients.Compensatory increase of Foxp3 mRNA was found in IRH (0.124(0.073-0.198) vs 0.071 (0.046-0.118),P <0.05).The expression of Bcl-6 was higher(2.243(0.854 4.544)vs 1.211 (0.131-2.816),P <0.05).Compared to aplastic anemia,the expression of RORγt was lower in IRH (0.133 (0.068-0.189) vs 0.290 (0.138-0.480),P < 0.01) and the ratio of Treg/Th17 shifted to Th17 in patients with aplastic anemia(Foxp3/ RORγt ratio,0.500(0.240-0.795)vs 0.975 (0.483-1.416),P<0.01).Conclusion As one kind of bone marrow failures caused by autoantibody to bone marrow cells,IRH may occur due to a high expression of Bcl-6 in CD4 + T cells,its immunopathogenesis is different from that of aplastic anemia.
More- 浏览:533
- 被引:6
- 下载:144
相似文献
- 中文期刊
- 外文期刊
- 学位论文
- 会议论文