高分辨熔解曲线分析在肿瘤分子检测中的应用
High-resolution melting analysis:applications in molecular tumor testing
高分辨熔解曲线分析具有检测成本低、操作简便的特点,且闭管操作无交叉污染的风险,已经成为临床诊断的一大有力工具,尤其在肿瘤的分子检测中已经得到了广泛使用,检测手段包括突变检测、测序前筛查、甲基化程度分析、拷贝数变异分析等,涵盖了肿瘤筛查、诊断、个体化治疗、预后判断等各个阶段。利用高分辨熔解曲线分析具有较高检测灵敏度的特点,与测序技术相结合可以显著提高分子检测的准确性,同时大大降低检测成本,为分子检测技术的发展开拓了新的空间。(中华检验医学杂志,2017,40:80-83)
更多High-resolution melting technique shows great potential as a clinical diagnostic tool because of its cost-effectiveness , convenience and closed-tube format which can prevent the risk of cross contamination.HRM analysis has been widely used in molecular diagnostics in the management of cancer by mutation detection , pre-sequence screening , methylation and copy number variations , which involves cancer screening, diagnosis, individual treatment and prognosis .Take advantage of the high detection sensitivity of HRM, the accuracy of mutation screening method could be improved and the test cost could be reduced by combing HRM analysis with sequencing , which helps to develop the molecular diagnostic technique .
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